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Figure 4 | Breast Cancer Research

Figure 4

From: Analysis of EpCAM positive cells isolated from sentinel lymph nodes of breast cancer patients identifies subpopulations of cells with distinct transcription profiles

Figure 4

Expression results from 10 EMT arrays representing only EpCAM+ cell populations. The first five samples (G+, L+; 544+, A+ and 517+) also expressed hMAM while the last five (546+, 540+, 542+, P+ and Q+) were negative for hMAM as determined by RT-PCR. Fold changes were calculated relative to the average expression of each gene. a) All hMAM expressing cell populations co-expressed other epithelial transcripts as E-cadherin (CDH1), desmoplakin (DSP), and cytokeratins 7 and 19 (KRT7/19). The estrogen receptor (ESR1) and ERBB3 (HER3) were also expressed mainly by hMAM positive cells. b) In contrast, EpCAM+/hMAM- cells show increased expression of EMT markers; the E-cadherin repressor Snai2, the mesenchymal filament vimentin (Vim) and Wnt5B, a member of the wnt-signaling pathway, were all increased in EpCAM+/hMAM- compared with EpCAM+/hMAM+ cell populations.

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