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Table 5 Antibodies used during Western blot analysesa

From: Allele-specific regulation of FGFR2 expression is cell type-dependent and may increase breast cancer risk through a paracrine stimulus involving FGF10

Antigen targeted Antibody (catalog number, manufacturer) Clonality Dilution Origin
Primary antibodies     
   FGFR2 Bek C-17 (sc-122, SZ) Polyclonal 1 in 500 Rabbit
   α-tubulin Anti-α-tubulin (T6199, SA) Monoclonal 1 in 8,000 Mouse
   ERK1/2 p44/42 MAPK antibody (9102, CS) Polyclonal 1 in 100 Rabbit
   Phosphorylated ERK1/2 Phospho-p44/42 MAPK antibody (4377, CS) Monoclonal 1 in 200 Rabbit
   FRS2α FRS2 H-91 (sc-8318, SZ) Polyclonal 1 in 50 Rabbit
   Phosphorylated FRS2α Phospho-FRS2-α antibody (3864, CS) Polyclonal 1 in 50 Rabbit
Secondary antibodies     
Mouse IgG IRDye 680 anti-mouse IgG (926-32220, LC) Polyclonal 1 in 7,500 Goat
Rabbit IgG IRDye 800CW anti-rabbit IgG (926-32211, LC) Polyclonal 1 in 5,000 Goat
  1. aFGFR2: fibroblast growth factor receptor 2 gene; ERK1/2: extracellular signal-regulated kinases 1 and 2; FRS2α: fibroblast growth factor receptor substrate 2α; IgG: immunoglobulin G; MAPK: mitogen-activated protein kinase. Manufacturer names are abbreviated as follows: SZ: Santa Cruz Biotechnology, Santa Cruz, CA, USA; SA: Sigma-Aldrich, Zwijndrecht, The Netherlands; CS: Cell Signaling Technology, Danvers, MA, USA; LC, LI-COR Biosciences, Cambridge, UK. For each antibody, the target, manufacturer, clonality, dilution used during Western blot analysis and origin are given.