Figure 5
Expression of 14-3-3ζ and associated signature genes in 14-3-3ζ knockdown MCF-7 cells. (a) Real-time PCR for six signature genes in control (Ctrl), 14-3-3ζ knockdown (KD) and KD cells with reexpression of 14-3-3ζ (KD_R). Reduced levels of 14-3-3ζ correlated with decreased levels of all investigated genes (black bars; *** P < 0.001, ** P < 0.01). 14-3-3ζ KD cells with re- expression of 14-3-3ζ (hatched bars) showed gene expression similar to the control (Ctrl) cells. 14-3-3ζ KD was also associated with decreased FOXM1 mRNA that was reversed with reexpression of 14-3-3ζ. (b) 14-3-3ζ and FOXM1 protein in Ctrl, 14-3-3ζ KD, and with 14-3-3ζ KD and reexpression. Western blots are shown with actin as loading control. (c) Impact of siFOXM1 treatment (black bars) on FOXM1 and expression of 14-3-3ζ and 14-3-3ζ signature genes. (d) Re-expression of FOXM1(+FOXM1) in Ctrl and 14-3-3ζ KD cells and its effect on 14-3-3ζ and signature genes. Minus indicates no added FOXM1.