Sunitinib inhibited VEGF-C-induced functions of LECs. (a) Quantification of LEC migration induced by VEGF-C in cells treated with DMSO or 10 nmol/ml of sunitinib. Relative migration indicates the number of migrated cells normalized to the number of control cells. Columns indicate the values from four experiments; bars, SD. *P < 0.05 as compared with control. Sunitinib suppressed VEGF-C-induced LEC migration. (b) Photographs of VEGF-C-induced tube formation in cells treated with sunitinib or DMSO (control). VEGF-C promoted cord formation after overlay of type I collagen, which was suppressed by sunitinib treatment, but not by anti-VEGFR2 antibody. The black line represents 200 μm. (c) Quantification of tube formation. Columns indicate the values from the data of four fields; bars, SD. Sunitinib inhibited VEGF-C-induced tube formation. *P < 0.05 as compared with control. DMSO, dimethyl sulfoxide; LEC, lymphatic endothelial cell; Suni, sunitinib; VEGF, vascular endothelial growth factor; VEGFR, vascular endothelial growth factor receptor.