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Figure 3 | Breast Cancer Research

Figure 3

From: Analysis of tumor environmental response and oncogenic pathway activation identifies distinct basal and luminal features in HER2-related breast tumor subtypes

Figure 3

Subgroup 7 tumors are characterized by increased levels of hypoxia-related gene expression. Subgroup 7 tumors express higher levels of hypoxia-induced genes as compared to subgroup 10 tumors in the (A) primary and the (B) validation datasets. Subgroup 7 tumors express significantly higher levels of HIF-1α mRNA in the (C) primary dataset (P <0.0001) and (D) the validation dataset (P = 0.0016) when compared to subgroup 10 tumors as examined by microarray analysis. (E) Subgroup 7 representative cell line AU565 (P <0.0001) demonstrated higher levels of HIF-1α expression as compared subgroup 10 cell line, HCC202 when examined by RT-PCR under normal (21% O2) and hypoxic (1% O2) conditions. Similarly, significantly higher expression of EPAS1 (HIF-2α) was noted in the subgroup 7 in (F) the primary dataset (P <0.0001) and (G) the validation dataset (P <0.0001) when compared to subgroup 10 tumors by microarray analysis. AU565 (P <0.0001) demonstrated higher levels of EPAS1 (HIF2α) expression as compared subgroup 10 cell line, HCC202, when examined by RT-PCR under normal (21% O2) and hypoxic (1% O2) conditions.

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Breast Cancer Research

ISSN: 1465-542X

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