Figure 1From: Epithelial to mesenchymal transition markers expressed in circulating tumour cells of early and metastatic breast cancer patientsTwist and vimentin expression in HeLa cells spiked in blood of normal volunteers. ARIOL system images of HeLa cells spiked in the blood of normal volunteers. (A) Row I: Positive control for Twist. HeLa cells were stained with pan-CK A45-B/B3 antibody/secondary FITC anti-mouse antibody (green)/Twist anti-rabbit antibody/Alexa Fluor 555 anti-rabbit antibody (orange). Row II: Negative control for Twist. Cells were stained with pan-CK A45-B/B3 antibody/FITC anti-mouse (green) and Alexa Fluor 555 IgG isotype antibody. Cell nuclei were stained with DAPI (blue). Original magnification, x400. (B) Row I: Positive control for vimentin. Cells were stained with pan-CK A45-B/B3 antibody/FITC anti-mouse antibody (green)/vimentin anti-rabbit antibody/Alexa Fluor 555 anti-rabbit antibody (orange). Row II: Negative control for vimentin. Cells were stained with pan-CK A45-B/B3 antibody/FITC anti-mouse antibody (green) and Alexa Fluor 555 IgG isotype antibody. Row III: Negative control for pan-CK. Cells were stained with FITC IgG isotype antibody/vimentin anti-rabbit antibody/Alexa Fluor 555 anti-rabbit antibody (orange). Cell nuclei were stained with DAPI (blue). Original magnification, x400. ARIOL system = automated image analysis system; CK = cytokeratin; FITC = fluorescein isothiocyanate; HeLa = patient with cervical adenocarcinoma from which the cell line was derived; IgG = immunoglobulin G; DAPI = 4',6-diamidino-2-phenylindole.Back to article page