Sulfotransferase gene | Pearson r | number | Pvalue (two-tailed) |
---|
CHST3
| 0.25 | 16 | 0.36 |
CHST7
| 0.45 | 12 | 0.14 |
CHST11
|
0.85
|
16
|
<0.0001
|
CHST12
| 0.32 | 12 | 0.30 |
CHST13
| -0.37 | 12 | 0.24 |
CHST14
| 0.20 | 12 | 0.52 |
CHST15
| 0.29 | 16 | 0.31 |
- Gene expression was measured by qRT-PCR and normalized using 18S transcript. Mean Fluorescence Intensities of P-selectin binding to cells was assayed by flow cytometry. Cell lines were MCF7, MDA-MB-468, MDA-MB-231, and MDA-MET. Each assay was repeated independently 3 times per cell line for CHST7, CHST12, CHST13 and CHST14 and 4 times per cell line for CHST3, CHST11, CHST15. Then the data were log transformed and subjected to correlation analysis using both Pearson (parametric) and Spearman (nonparametric) correlation tests in order to check validity of assumptions. Correlation coefficients were similar, assumptions were considered valid, and the Pearson coefficient was reported. CHST3, Carbohydrate (chondroitin 6) sulfotransferase 3; CHST7, Carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 7; CHST11, Carbohydrate (Chondroitin 4) Sulfotransferase 11; CHST12, Carbohydrate (Chondroitin 4) Sulfotransferase 12; CHST13; Carbohydrate (Chondroitin 4) Sulfotransferase 13; CHST14, Carbohydrate (N-acetylgalactosamine 4-O) Sulfotransferase 14; CHST15, Carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) Sulfotransferase 15.