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Table 1 Primer and siRNA sequences used in this study

From: Chondroitin sulfates play a major role in breast cancer metastasis: a role for CSPG4 and CHST11gene expression in forming surface P-selectin ligands in aggressive breast cancer cells

Primer

Sequence

18S forward

18S reverse

5' TTCGAACGTCTGCCCTATCAA 3'

5' ATGGTAGGCACGGCGACTA 3'

β-actin forward

β-actin reverse

5' GACTTCGAGCAAGAGATGGCCAC3'

5' CAATGCCAGGGTACATGGTGGTG3'

CHST3 forward

CHST3 reverse

5' GACTTTGTGCACAGCCTGAA 3'

5' AGAGCTTGGGGAATCTGCTT 3'

CHST7 forward

CHST7 reverse

5' GGGGCAATCTGTCACACTCT 3'

5' AATTGCACAGCAGTTGTTGG 3'

CHST11 forward

CHST11 reverse

5' TCCCTTTGGTGTGGACATCT 3'

5' CACGTGTCTGTCACCTGGTC 3'

CHST12 forward

CHST12 reverse

5' GCACACGTCCTTCTCTAGGC 3'

5' AAACTCGTCGACATCGGAGT 3'

CHST13 forward

CHST13 reverse

5' CCGGCATTTGGAAACAGAG 3'

5' GGGTCCTGATCCAGGTCATA 3'

CHST14 forward

CHST14 reverse

5' TCTGATCCCCCATTTATCCA 3'

5' GGAGCAGAAAGGCACAAAAG3'

CHST15 forward

CHST15 reverse

5' TGAGTTCACGACCAGACAGC 3'

5' CGTACCAACAGGGGCTCTTA 3'

CSPG4 forward

CSPG4 reverse

5' CACACAGAGGAACCCTCGAT 3'

5' CTTCAGCGAGAGGAGCACTT 3'

ER1 forward

ER1 reverse

5' AGGTGGGATACGAAAAGACCG 3'

5' AAGGTTGGCAGCTCTCATGTC 3'

GAPDH forward

GAPDH reverse

5' ACAGTCAGCCGCATCTTCTT 3'

5' ACGACCAAATCCGTTGACTC 3'

NRP-1 forward

NRP-1 reverse

5' CAAGGTGTTCATGAGGAAGTTCAA 3'

5' CCGCAGCTCAGGTGTATCATAGT 3'

SDC-1 forward

SDC-1 reverse

5' GCTCTGGGGATGACTCTGAC 3'

5' GGTCTGCTGTGACAAGGTGA3'

SDC-4 forward

SDC-4 reverse

5' GTCTGGCTCTGGAGATCTGG3'

5' CACCAAGGGATGGACAACTT3'

siRNA

Sequence

#31

#32

#33

UCAGUAGUUCUCGUAGACUtt

UUCUGGGUGAACUUGUUGCgg

AUCGAGUUUGUAGACUUCGta

  1. 18S, 18S ribosomal RNA; CHST3, Carbohydrate (chondroitin 6) sulfotransferase 3; CHST7, Carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 7; CHST11, Carbohydrate (Chondroitin 4) Sulfotransferase 11; CHST12, Carbohydrate (Chondroitin 4) Sulfotransferase 12; CHST13, Carbohydrate (Chondroitin 4) Sulfotransferase 13; CHST14, Carbohydrate (N-acetylgalactosamine 4-O) Sulfotransferase 14; CHST15, Carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) Sulfotransferase 15; CSPG4, Chondroitin Sulfate Proteoglycan 4; ER1, Estrogen Receptor 1; GAPDH, Glyceraldehyde-3-phosphate dehydrogenase; NPR-1, Neuropilin-1; SDC-1, Syndecan-1; SDC-4, Syndecan-4; siRNA, short interfering RNA.