Enzymatic removal of surface CS-GAGs reduced lung colonization of 4T1 cells. 4T1 cells were treated with chondroitinase ABC or buffer and injected into the tail vein of BALB/c mice (10 per group). Mice were sacrificed 25 days later and the number of metastases to the lung was measured by clonogenic assay and expressed as "Lung Metastases". Boxes show medians and quartiles while whiskers show ranges; plus signs indicate means. P = 0.0002 by Wilcoxon rank-sum test.