Figure 6
Geminin overexpression promotes TopoIIα deSUMOylation on chromosomes. (A) Expression in total cell extracts of the indicated proteins in HME or induced Gem9 cells. (B) Coimmunoprecipitates of the indicated proteins using anti-TopoIIα antibodies from whole cell extracts. Top image shows reprobing of the TopoIIα immunoprecipitate with anti-Pan SUMO antibody. (C) Coimmunoprecipitates of the indicated proteins with anti-Sp1 or anti-geminin antibodies (left) or TopoIIα (right) from the chromatin of HME or induced Gem9 cells. (D) Expression of the indicated proteins in HME or induced Gem9 cells (72 hours). (E) Coimmunoprecipitates of the indicated proteins with anti-geminin antibody from the chromatin of G2/M, M or M/G1 HME or induced Gem9 cells (72 hours). (F) Expression of the indicated proteins in whole cell extracts of G2/M, M or M/G1 HME or induced Gem9 cells (72 hours). IP = immunoprecipitation; IB = immunoblotting.