Down-regulation of TrkB contributes to miR-200c mediated suppression of anoikis resistance. Cells were transfected with empty vector (EV) (left) or TrkB (right) and 24 hrs with miRNA constructs. Twenty-four hours later cells were plated on poly-HEMA coated plates and cell death ELISA performed at time points indicated (a) and (c). Columns, mean of three biological replicates, bars, standard deviation of the mean. ANOVA, * P < 0.05, ** P < 0.01. (b) and (d) Immunoblot for TrkB and α-tubulin (loading control).