Down-regulation of MSN contributes to miR-200c mediated suppression of migration. Cells were transfected with empty vector (EV) or MSN and 24 hrs later with miRNA constructs. BT549 (left) and Hec50 (right) cells were treated with mitomycin C and given 24 hrs to migrate. (a) Brightfield images of crystal violet stained cells, dashed black lines indicate edges of the wound immediately after wounding. Scale bars are 100 μm. (b) Quantitation of migratory ability of cells. Columns, mean of five replicates, bars, standard deviation of the mean. ANOVA, * P < 0.05, ** P < 0.01, Tukey-Kramer post-hoc test, ΦΦ P < 0.01. (c) Immunoblot for MSN, E-cadherin and α-tubulin (loading control).