Moesin ( MSN ), a regulator of cortical actin-membrane binding, is directly targeted and down-regulated by miR-200c. (a) Regions of the 3' UTR where miR-200c is predicted to bind. Hec50 cells treated with transfection reagent only (mock), scrambled negative control (neg), miR-200c mimic (200c), miR-200c antagomiR alone (α200c) or in conjunction with miR-200c (α200c + 200c) and luciferase assay performed. Columns, mean of five replicates, bars, standard deviation of the mean. ANOVA with Tukey-Kramer post-hoc test, ** P < 0.01. (b) Immunoblot for MSN and α-tubulin (loading control) expression.