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Figure 3 | Breast Cancer Research

Figure 3

From: Quantitative assays for the measurement of HER1-HER2 heterodimerization and phosphorylation in cell lines and breast tumors: applications for diagnostics and targeted drug mechanism of action

Figure 3

Cell line panel in HER1-HER2 suite of activated FFPE assays and co-IP. Cell lines were mock-stimulated or stimulated with 10 nM EGF for 10 minutes, then harvested and either lysed or fixed and paraffin-embedded. Cartoons to the left of each graph depict the configuration of the antibodies in the corresponding VeraTag™ FFPE assay. Gray bars represent normalized RPA from unstimulated and black bars represent signal from EGF-stimulated cells. Duplicate CE injections were measured. Corresponding co-IP assays are to the right of each FFPE assay and all cell lines were assayed in the same experiment. A) HER1-HER2. B) pHER1-HER2. C) pHER1(1173). D) pHER1(pan). <E) pHER2(pan).

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