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Figure 1 | Breast Cancer Research

Figure 1

From: Quantitative assays for the measurement of HER1-HER2 heterodimerization and phosphorylation in cell lines and breast tumors: applications for diagnostics and targeted drug mechanism of action

Figure 1

Depiction of VeraTagâ„¢ technology. In both lysate and FFPE assays, one antibody conjugated to biotin and one antibody conjugated to a VeraTagâ„¢ reporter bind to their respective analytes. Streptavidin-functionalized sensitizer dye binds to Ab-biotin. 670 nm light causes singlet oxygen release, which induces cleavage and release of the VeraTagâ„¢ reporter molecule into illumination buffer (IB). IB is collected and analyzed by capillary electrophoresis. For details, see Materials and methods.

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