Skip to main content
Figure 1 | Breast Cancer Research

Figure 1

From: Quantitative assays for the measurement of HER1-HER2 heterodimerization and phosphorylation in cell lines and breast tumors: applications for diagnostics and targeted drug mechanism of action

Figure 1

Depiction of VeraTag™ technology. In both lysate and FFPE assays, one antibody conjugated to biotin and one antibody conjugated to a VeraTag™ reporter bind to their respective analytes. Streptavidin-functionalized sensitizer dye binds to Ab-biotin. 670 nm light causes singlet oxygen release, which induces cleavage and release of the VeraTag™ reporter molecule into illumination buffer (IB). IB is collected and analyzed by capillary electrophoresis. For details, see Materials and methods.

Back to article page