Figure 1From: Quantitative assays for the measurement of HER1-HER2 heterodimerization and phosphorylation in cell lines and breast tumors: applications for diagnostics and targeted drug mechanism of actionDepiction of VeraTagâ„¢ technology. In both lysate and FFPE assays, one antibody conjugated to biotin and one antibody conjugated to a VeraTagâ„¢ reporter bind to their respective analytes. Streptavidin-functionalized sensitizer dye binds to Ab-biotin. 670 nm light causes singlet oxygen release, which induces cleavage and release of the VeraTagâ„¢ reporter molecule into illumination buffer (IB). IB is collected and analyzed by capillary electrophoresis. For details, see Materials and methods.Back to article page