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Figure 5 | Breast Cancer Research

Figure 5

From: Estrogen receptor β represses Akt signaling in breast cancer cells via downregulation of HER2/HER3 and upregulation of PTEN: implications for tamoxifen sensitivity

Figure 5

PTEN levels increase upon ERβ expression. (A) T47-DERβ cells, MCF-7ERβ cells and T47-DPBI control cells were treated for 4 days with 10 ng/mL or 0.01 ng/mL doxycycline in the presence or absence of 10 nM DPN, and lysates were analyzed by immunoblotting. (B) Phosphatase and tensin homologue deleted on chromosome 10 (PTEN) immunofluorescence of T47-DERβ cells treated for 4 days with 10 ng/mL (-ERβ) or 0.01 ng/mL (+ERβ) doxycycline. Scale bar, 10 μm. (C) T47-DERβ cells were treated for 4 days with 10 ng/mL (-ERβ) or 0.01 ng/mL (+ERβ) doxycycline in the presence or absence of 10 nM DPN with or without 100 nM ICI or 100 nM 4-OH-T, and lysates were analyzed by immunoblotting.

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Breast Cancer Research

ISSN: 1465-542X

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