Mrg15 deficiency impairs Rad51 foci formation and reduces Brca1 and Brca2 levels. (a) Left panel: number of cells with Rad51 nuclear foci (>4 foci per nuclei) in wild-type and Morf4l1-/- murine embryonic fibroblast (MEF) clones after (16 hours) treatment with 10 Gy. **Significant difference (two-tailed t test, P < 0.001). Right panel: representative images of Rad51 and pS139-H2ax immunodetection in cultures counted above for foci. DAPI, 4,6-diamidino-2-phenylindole; IR, γ-irradiated. (b) Levels of Brca1, Brca2 and Rad51, and control Actb, in whole cell extracts of Morf4l1-/- MEFs and wild-type counterparts (three cell clones of each genotype are shown).