Figure 3

Synergistic induction of apoptosis by AR and mitogen-activated protein kinase kinase inhibitors in HCC-1954 and MDA-MB-453 cell lines. (A) Histogram showing the percentage of apoptosis (P1) in control (solvent-only treated) HCC-1954 cell line using annexin V-FITC flow cytometry. (B) Histogram showing the percentage of apoptosis following CI-1040 treatment at 20 μM (CI 20 μM) in HCC-1954 cell line. (C) Histogram showing the percentage of apoptosis following flutamide (FLU) treatment at 100 μM in HCC-1954 cell line. (D) Histogram showing the percentage of apoptosis following combination therapy with CI-1040 at 10 μM and flutamide at 20 μM in HCC-1954 cell line. Combination index (CI) is calculated using the concentrations of monotherapies with these agents as shown in Figures 3B and 3C that induced a level of apoptosis similar to that of combination therapy. (E) Histogram showing the percentage of apoptosis in control MDA-MB-453 cell line. (F) Histogram showing the percentage of apoptosis following CI-1040 treatment at 20 μM in MDA-MB-453 cell line. (G) Histogram showing the percentage of apoptosis following flutamide treatment at 80 μM in MDA-MB-453 cell line. (H) Histogram showing the percentage of apoptosis and CI following combination therapy with CI-1040 at 10 μM and flutamide at 20 μM in MDA-MB-453 cell line.