Skip to main content
Figure 6 | Breast Cancer Research

Figure 6

From: Estrogen receptor beta inhibits transcriptional activity of hypoxia inducible factor-1 through the downregulation of arylhydrocarbon receptor nuclear translocator

Figure 6

ERβ decreases the hypoxic induction of VEGF by reducing the recruitment of HIF-1 to the hypoxia-dependent VEGF promoter. (a) Hep3B cells in six-well plates were transfected with hERβ (2 μg). At 24 h post-transfection, cells were incubated for 24 h under normoxic or hypoxic conditions. Total RNA from untransfected Hep3B cells and cells transfected with hERβ plasmids were analyzed using VEGF-specific primers, as described in the Materials and Methods. Values represent the mean ± S.D. (N = 3). * P <0.05. All experiments were repeated at least three times. (b) Hep3B cells were transfected with hERβ using the PEI method. At 24 h post-transfection, cells were incubated for 24 h under hypoxic conditions. After incubation, cells were subjected to ChIP analysis with HIF-1α or control IgG antibody. Shown here are immunoenriched DNA samples amplified using conventional PCR (top) and quantified results by qPCR (bottom) for an HIF-1α binding site on the VEGF promoter from three separate experiments.

Back to article page