Figure 3

Effects of ERβ on ARNT and CYP1A1 expression level. (a) PC3 cells in six-well plates were transfected with control siRNA or ERβ SMARTpool siRNA. At 48 h post-transfection, total protein extracts were immunoblotted as indicated (left panel). Knockdown of ERβ mRNA was also verified by RT-qPCR. Both quantified and gel electrophoretic results of qPCR products are shown (right panel). * P <0.001. (b) MCF7 cells in six-well plates were transfected with hERβ (2 μg). At 24 h post-transfection, cells were treated for 24 h with 1 nM dioxin as indicated. Total RNA were extracted and expression of CYP1A1 was analyzed by qPCR. * P <0.0005.