JAM-A knockdown reduces breast cancer cell adhesion and migration. (a) Transfection of MCF7 breast cancer cells with short interfering RNA (siRNA) targeting JAM-A reduces JAM-A protein expression. (b) Adhesion of MCF7 cells transfected with JAM-A siRNA to a fibronectin substrate was reduced relative to that induced under control conditions. (c) Individual cell migration of MCF7 cells transfected with JAM-A siRNA or control siRNA MCF7 cells across fibronectin-coated, 8-μm pore filters was reduced relative to control conditions. Images show DAPI (4'-6-diamidino-2-phenylindole)-stained migrated cells on transwell filters. Error bars refer to standard deviation of pooled triplicate experiments. JAM-A, junctional adhesion molecule-A.