Figure 6
From: Mechanisms of suberoylanilide hydroxamic acid inhibition of mammary cell growth
Effect of SAHA on cyclin-associated kinase activities in TM10 and TM2H cells. Equal amounts of nuclear protein extracts (250 μg) from TM10 and TM2H cells at control (C) and treated with 2.5 μM SAHA for 24 h (T) were immunoprecipitated with antibodies against anti-cyclin D1 (A), anti-cyclin E (B) and anti-cyclin A (C) or with rabbit preimmune serum, followed by kinase activity assay, as described in Materials and methods. The negative control in each panel was 250 μg protein extract from TM2H cells at control condition immunoprecipitated with rabbit preimmune serum. The phosphorylated substrates, pRb and histone (H1), were scanned and quantitated densitometricaly using a phosphoimager analyzer. Histograms represent arbitrary units of the phosphorylated substrates after subtracting the background and the negative control.