Figure 5
From: Mechanisms of suberoylanilide hydroxamic acid inhibition of mammary cell growth
Effect of SAHA on phosphorylation levels of the pRb pocket proteins. Western blot analysis of 100 μg nuclear protein extracts of TM10 and TM2H cell lines in control (C) or treated with 2.5 μM SAHA for 24 h (T). Protein samples were resolved by 10% SDS-PAGE, transferred onto nitrocellulose membrane, blocked and probed with antibodies against pRb, p107 and Rb2/p130 as described in Materials and methods. Note the fast migrating and more intense Rb, p107 and Rb2/p130 'hypophosphorylated forms' in SAHA treated (T) versus control (C) cell lines.