Figure 5From: During hormone depletion or tamoxifen treatment of breast cancer cells the estrogen receptor apoprotein supports cell cycling through the retinoic acid receptor α1 apoproteinERBB2 and ERBB3 mRNA levels following depletion of apo-ER or apo-RARα1 in MCF-7 cells. Cells were treated with vehicle, E2 (1 nM) or transfected with control siRNA, ER siRNA or RARα siRNA. Ninety-six days later the cells were harvested to extract total RNA for the measurement of ERBB2 and ERBB3 by real time RT-PCR. Values are normalized to the GAPDH values. P-values for the differences noted in the text were ≤0.001.Back to article page