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Table 1 Cytokine secretion in antigen-stimpulated splenocytes from MMTV.f.huHER2(Fo5) transgenic mice immunized with anti-idiotype scFv 69 vaccine

From: Vaccination with human anti-trastuzumab anti-idiotype scFv reverses HER2 immunological tolerance and induces tumor immunity in MMTV.f.huHER2(Fo5) mice

Cytokine

Conc

HER2-transgenic mouse immunized with

Stimulating antigen

(μg/ml)

  
  

PBS

scFv69

Interleukin-2

   

Con A

7.5

356.9 ± 5.7

422.8 ± 26.5

Control scFv 13R4

2.0

<4

<4

ScFv69

2.0

<4

10.6 ± 0.8

Control Mfe23-Fc

2.0

<4

<4

HER2-Fc

2.0

<4

<4

Interferon-gamma

   

Con A

7.5

21.4 ± 0.9

24.2 ± 0.8

Control scFv 13R4

2.0

<1

7.0 ± 3.6

ScFv69

2.0

<1

17.0 ± 3.7

Control Mfe23-Fc

2.0

<1

<1

HER2-Fc

2.0

<1

<1

  1. *ng/ml.
  2. Cytokine (Il-2 and IFN-gamma) secretion in splenocytes from MMTV.f.huHER2(Fo5) transgenic mice immunized with PBS or anti-Id scFv69. Splenocytes were isolated on Day 42 post-immunization. Supernatants from antigen-stimulated cells were recovered 24 h (for IL-2) and 72 h (for IFN-gamma) after in vitro stimulation. IL-2 and IFN-gamma detection (ng/ml) were performed using an ELISA assay, with a standard curve obtained with recombinant IL-2 (detection limit 4 ng/ml) or recombinant IFN-gamma (detection limit 1 ng/ml). Results are the mean ± SD of triplicate wells and are representative of two independent experiments. Mfe23-Fc and scFv13R4 are two irrelevant antigens. Con A, Concanavalin A.
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Breast Cancer Research

ISSN: 1465-542X

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