p53β and p53γ mRNA are differentially expressed in primary breast tumours from patient to patient. Total RNA from 127 primary breast tumours were provided by the Tayside Tissue Bank. RNA quality was assessed, and reverse transcription was performed as described in Materials and methods. The p53 cDNA were amplified by polymerase chain reaction (PCR) assays using primers specific for p53, p53β and p53γ, as shown in Table S1 in Additional file 1. Amplification of actin cDNA by PCR assay was used as a positive control. Tumour sample numbers are indicated. C, negative control; M, molecular marker.