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Figure 2 | Breast Cancer Research

Figure 2

From: α-Tocopheryloxyacetic acid: a novel chemotherapeutic that stimulates the antitumor immune response

Figure 2

Effect of α-TEA on activation status of tumor-infiltrating T cells. BALB/c mice with established 4T1 mammary tumors (day 10) received oral α-TEA in their diet. On day 18 (D18) and day 26 (D26) posttumor injection, tumor-infiltrating immune cells (TICs) were isolated and analyzed by polychromatic flow cytometry. (A) After applying a live cell gate, CD3+ cells were identified by the R1 gate. Subsequently, CD8+ and CD4+ T cells were delineated with the R2 and R3 gates and were then further interrogated for CD62L (R4) and CD44 (R5) expression. On the basis of the defined positive staining regions, combination function analysis (FCOM) calculated four phenotypes (FCOM region array). Activated T cells were identified as CD4+CD44+CD62L- or CD8+CD44+CD62L-. Representative FCOM arrays from CD8+ T cells are shown. (B) TICs were analyzed from three individual mice in each of two independent experiments. Subphenotype frequencies are based on all live tumor-infiltrating cells.

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