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Table 2 Regulated mRNAs in BC cells after miR-2 1 knockdown determined by oligo array

From: Knockdown of miR-21 in human breast cancer cell lines inhibits proliferation, in vitro migration and in vivotumor growth

     Fold change  
Gene Name NCBI accession number Genbank accession number Description MCF-7 MDA-MB-231 Prediction program
ANKRD46 NM_198401 U79297, BC035087 ankyrin repeat domain 46 1.78 2.57 # miRBase Targets V5
ACTN4 NM_004924 BC005033 Alpha-actin 4 (F-actin cross linking protein) 1.75 1.55  
NR0B1 NM_000475 S74720 Nuclear receptor 0B1 (Nuclear receptor DAX-1) 1.60 1.32  
HIRA NM_003325 BC039835, X89887 HIRA protein 1.48 1.32  
COL18A1 NM_030582, NM_130444, NM_130445 AF018081 Collagen alpha 1(XVIII) chain precursor 1.43 1.35  
SAMD11 NM_152486 BC024295, AK054643 sterile alpha motif domain containing 11 1.42 1.34  
EIF4A2 NM_001967 AL117412 Eukaryotic initiation factor 4A-II 1.42 1.41 miRNAMap
SFRS16 NM_007056 AF042800, AK094681 Splicing factor, arginine/serine-rich 16 1.40 1.34  
NP_079316 - BC004930 zinc finger protein 614 1.37 1.36  
IL18BP NM_173043, NM_005699, NM_173042 AF110801 Interleukin-18 binding protein precursor 1.36 1.34  
GBF1 NM_004193 AK025330, AF068755 Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 1.35 1.51  
GNPDA1 NM_005471 AJ002231 Glucosamine-6-phosphate isomerase 1.34 1.32  
SPUF_HUMAN NM_013349 - SPUF protein precursor 1.34 1.31  
PLXNA1 NM_032242 AK127254 plexin A1; plexin 1 1.32 1.57  
STMN3 NM_015894 AK094112 Stathmin 3 (SCG10-like protein) 1.32 1.47  
NP_056236 NM_015421 AK093383, AL080088 DKFZP564K2062 protein 1.31 1.33  
- - AK056473 - 1.31 1.30  
AP2B1 NM_001282 M34175 adaptor-related protein complex 2, beta 1 subunit 1.31 1.45  
HIST1H2AC - CR608156 Histone H2A.l (H2A/l) -1.47 -1.67  
PRKCI NM_002740 L33881, BC042405, BC022016 Protein kinase C, iota type -1.43 -1.32  
SLC25A3 NM_002635, NM_213611, NM_213612, NM_005888 BX647062, AK057575 Phosphate carrier protein, mitochondrial precursor (PTP) -1.39 -1.39  
CCNE1 NM_057182, NM_001238 M74093, BC035498 G1/S-specific cyclin E1 -1.37 -1.39  
DCAMKL1 NM_004734 AB002367 Serine/threonine-protein kinase DCAMKL1 -1.34 -1.46  
GNG12 NM_018841 AL832431 Guanine nucleotide-binding protein G(I)/G(S)/G(O) gamma-12 subunit -1.32 -1.40 Miranda
Q8TAY7 NM_024869 BC025658 hypothetical protein FLJ14050 -1.32 -1.31  
NP_653284 NM_144683 BC015582 hypothetical protein MGC23280 -1.31 -1.35  
NP_055182 NM_014367 - growth and transformation-dependent protein -1.30 -1.34  
  1. Fold-regulation of mRNAs affected by miR-21 knockdown compared with control-transfected cells (MCF-7 and MDA-MB-231) at indicated time points. Fold-changes for all mRNAs are derived from a single microarray experiment, with two relevant genes confirmed by qRT-PCR (Figure 4B). Shown are mRNAs changing by at least 1.3-fold in both cell lines. Genes in bold were selected for candidate gene analyses by qRT-PCR.; # marginal signal intensity; NCBI, National Center for Biotechnology Information; GB, GenBank.