mRNA profiling of miR-21-knockdown BC cell lines. MCF-7 and MDA-MB-231 cells were transfected with 50 nM LNA-antimiR-21 or LNA-control. Total RNAs were isolated from MCF-7 cells 48 h post transfection and from MDA-MB-231 cells 36 h post transfection, respectively, and were hybridized to the human genome oligo array V1.0 (CapitalBio, China). (a) The scatter plot shows the LNA-antimiR-21 expression values normalized to LNA-control values, applying a cut-off of 1.3. Each dot represents one probe set. (b) Unsupervised hierarchical cluster analysis of intersection of mRNAs differentially expressed in MCF-7 and MDA-MB-231 cells upon LNA-antimiR-21 treatment. Rows, mRNAs; columns, biological samples. For each mRNA, red is expression higher than average expression across all samples, green is expression lower than average. (c) Validation of microarray results by qRT-PCR, normalizing on GAPDH RNA levels. MCF/A, MCF/LNA-antimiR-21; MCF/C, MCF/LNA-control; MDA/A, MDA/LNA-antimiR-21, MDA/C, MDA/LNA-control.