Combination treatments induce Yap nuclear translocation, associated with suppression of phosphorylation of Akt and Yap. Isolated cytosolic and nuclear fractions from MDA-MB-231 cells treated with α-TEA + doxorubicin (DOXO) or α-TEA + cisplatin (CDDP) were used to detect Yap tanslocation from cytosol to the nucleus by western blot analyses (a). MDA-MB-231 cells were treated with DOXO or CDDP alone or in combination with α-TEA for 24 hours. Protein levels of pAkt and pYap were determined by western blot analyses with total Akt and Yap serving as controls (b). MDA-MB-231 cells were pre-treated with Akt inhibitor wortmannin at 1 μM or dimethylsulfoxide for 2 hours followed by treatments with DOXO or CDDP for 24 hours. Protein levels of p73, pAkt and pYap were determined by western blot analyses (c). Data are representative of at least two independent experiments. GAPDH, glyceraldehyde-3-phosphate dehydrogenase; VEH, vehicle.