Figure 6

CAMKK6b upregulates MMP-9 mRNA expression and ETS1 phosphorylation. (a) Effect of CAMKK6b on MMP-9 mRNA expression. MCF-7 cells were transiently transfected with empty vector or CAMKK6b plasmid for 24 hours. Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) analyses were performed to examine the expression of MMP-9 mRNA in vector- and CAMKK6b-transfected MCF-7 cells. The MMP-9 mRNA expression was normalized to levels of 18s ribosomal RNA. *P < 0.05 versus vector-transfected cells. The figure is a representative real-time RT-PCR analysis from three independent studies. (b) Effect of CAMKK6b on ETS-1 phosphorylation. MCF-7 cells were transiently transfected with empty vector or CAMKK6b plasmid. Phosphorylation of ETS1 (p-ETS1) in vector- and CAMKK6b-transfected cells was analyzed by Western blot analysis. Expression of total ETS1 (t-ETS1) was used as loading control. The figure is a representative Western blot from three independent studies. MMP, matrix metalloproteinase.