Figure 6

Deficiency of Mena alters mammary gland development. A. Representative images of mammary gland whole mounts from PyMT Mena WT, Het and Mena Null mice at 6 weeks (i) and 10 weeks (ii) of age. Scale bar = 1 cm. Enlarged area of six-week whole mount mammary glands shown in iii. In iii = whole mount mammary gland from PyMT Mena WT mouse. Black arrows point to examples of terminal end buds. Black arrowheads point to examples of branches. Images taken at 10×. B. Quantification of terminal end buds from mammary gland whole mounts. Examples of terminal end buds counted are shown in Figure 6A by the black arrows. N = 5 animals per genotype at each time point. P-values listed above bars. Error bars = SEM. C. Quantification of mammary duct branching quantified under 5×. Examples of branches counted are shown in Figure 6A iii by the black arrowheads. N = 5 fields/animal, 10 animals per genotype. P-values listed above bars. Error bars = SEM. D. Quantification of the length of the three longest ducts from mammary gland whole mounts. N = 10 animals per genotype. P-values listed above bars. Error bars = SEM.