Figure 5

Tumors from orthotopic transplantation of Mena Null cells show decreased intravasation and lung metastasis. A. Average number of circulating tumor cells in the blood of SCID mice injected with PyMT Mena WT, and Null tumor cells. N = 10 mice/genotype. P-values listed above bar. Error bars indicate SEM. B. Average number of spontaneous lung metastases in SCID mice injected with PyMT Mena WT, and Null tumor cells. N = 10 mice/genotype. P-values listed above bar. Error bars indicate SEM. C. Multiphoton microscopy of primary tumors of SCID mice injected with PyMT Mena WT, and Null tumor cells. Images taken at 20×. Blue = CFP tumor cells, Black = tumor stroma and vasculature. Scale bar = 50 μm.