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Figure 3 | Breast Cancer Research

Figure 3

From: Mena deficiency delays tumor progression and decreases metastasis in polyoma middle-T transgenic mouse mammary tumors

Figure 3

Deficiency of Mena dampens EGF-induced in vivo invasion and motility. A. In vivo invasion assay of PyMT mice with given Mena genotype. White bars indicate average number of tumor cells that invaded in the absence of EGF, gray bars indicate average number of tumor cells that invaded in the presence of 25 nM EGF. P-values listed above bars. N = 12 assays/genotype/condition. Error bars indicate SEM. B. Average number of cells moving/field quantified from intravital imaging of PyMT Mena WT, Het and Null mice. 30 to 50 fields analyzed from 10 mice per genotype. Error bars = SEM. C. Multiphoton microscopy of tumor cells moving within the primary tumors of PyMT Mena WT, Het and Null mice. Images taken at 60× over the course of 30 minutes. Yellow outlines indicate cells that are moving (refer to Additional files 2, 3, 4). White = CFP tumor cells, Green = GFP macrophages, Black = tumor stroma and vasculature. Scale bar = 50 μm.

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