Figure 8

Cell invasion and activity of MMP9 in HER2-positive BT474 breast cancer cells with suppressed FASN. (A) BT474 cells were treated with 50 ng/mL HRG or 50 ng/mL HRG plus 10 μM C75 for 36 hours. Cells shown in the microscopic image represent invasive cells. The changes in cell invasion compared with HRG-induced cell invasion are shown as a percentage in the bar graph. All experiments were done three times. A simple t-test was used to assess differences in the number of invaded cells between any two experimental conditions. *, statistically significant compared with cells treated with HRG; P < 0.05 was considered statistically significant. (B) BT474 cells were treated as described in Figures 7A, B and 8A, and the medium was harvested for zymogram assay as described in Materials and methods. MMP-9 activity is shown as the digested clear band in Coomassie blue-stained gel containing 0.2% gelatin.