Skip to main content
Figure 7 | Breast Cancer Research

Figure 7

From: Fatty acid synthase phosphorylation: a novel therapeutic target in HER2-overexpressing breast cancer cells

Figure 7

HRG-induced invasion in HER2-positive BT474 breast cancer cells treated with lapatinib or siRNA targeting FASN. (A) BT474 cells were treated with 50 ng/mL HRG or 50 ng/mL HRG plus 0.2 μM lapatinib for 36 hours. The invaded BT474 cells were stained with crystal violet and counted under a microscope. Representative microscopic images are shown. The changes in cell invasion compared with HRG-induced cell invasion are shown in a bar graph. All experiments were done three times. A simple t-test was used to assess differences in the number of invaded cells between any two experimental conditions. *, statistically significant compared with cells treated with HRG; P < 0.05 was considered statistically significant. (B) BT474 cells transfected with scrambled siRNA or siRNA targeting FASN (si-FASN) were treated with 50 ng/mL HRG for 36 hours. The efficiency of si-FASN knockdown was confirmed by Western blotting for FASN and β-actin (lower left). The changes in cell invasion compared with the invaded scrambled siRNA-transfected untreated cells are shown as a percentage in the bar graph (lower right). All experiments were done three times. A simple t-test was used to assess differences in the number of invaded cells between any two experimental conditions. *, statistically significant compared with scrambled si-RNA-transfected cells treated with HRG; P < 0.05 was considered statistically significant.

Back to article page