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Figure 5 | Breast Cancer Research

Figure 5

From: Fatty acid synthase phosphorylation: a novel therapeutic target in HER2-overexpressing breast cancer cells

Figure 5

FASN phosphorylation in breast cancer cells treated with C75. SKBR3 (A) and BT474 (B) cells were pretreated with 10 μM C75 for five hours and then treated for one additional hour with 1) no agent (control); 2) 50 ng/mL HRG; 3) 10 μM C75; or 4) 50 ng/mL HRG plus 10 μM C75. The immunoprecipitated FASN complexes were assessed for FASN, HER2 and PT66 by Western blotting. (C) Whole-cell lysates from SKBR3 and BT474 cells were subjected to Western blotting for pHER2 (Y1248), pHER3 (Y1289), pAkt (S473), pErk1/2, HER2, HER3, Akt, Erk1/2, FASN and β-actin.

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