Figure 4

FASN enzymatic activity of cells treated with or without lapatinib. FASN activity was measured in SKBR3 (A) and BT474 (B) cells treated with 50 ng/mL HRG, 1 μM lapatinib or 50 ng/mL HRG plus 1 μM lapatinib. Cells were harvested, and lipids were extracted as described in Materials and methods. A simple t-test was used to assess the differences in FASN activity between any two experimental conditions. *, statistically significant compared with FASN activity in control cells; ^#, statistically significant compared with FASN activity in cells treated with HRG; P < 0.05 was considered statistically significant.