Figure 1

Photograph of an actual margin after imaging and pathological methodology. (A) The six orange dots provide the location of inked sites and the four green dots denote the boundaries of the margin used for pathologic co-registration and diagnoses. Four margin dots (green) denote the corners of the measured margin while the eight orange dots denote the research dots (B). A transverse slice is taken from the specimen with the margins inked for orientation (C). Each research dot is embedded in a paraffin block from routine processing (D). A 5 μm section is taken from the paraffin block, stained and examined under a microscope by a specialized breast pathologist (E).