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Table 9 Percentage of label-retaining cells in vitro

From: Immortalized, premalignant epithelial cell populations contain long-lived, label-retaining cells that asymmetrically divide and retain their template DNA

  Percentage of cells expressing EdU
Cell type Passage 0 Passage 5
NSP2 17.4 14.7
NSP3 23.2 4.0
SP3 21.0 3.8
  1. The number of cells expressing EdU was counted in three random fields of view (100× magnification). In addition, the total number of cells in three random fields of view was determined (>100 cells total counted; 100× magnification). The total number of cells expressing EdU was then divided by the total number of cells in a field of view, and the percentage of cells expressing EdU per field of view was obtained.