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Table 7 Percentage of cells expressing an ER-α and 3H-TdR double-label per field of view found in the glands and mammary fat pads of Balb/C female mice that were either intact or implanted with immortalized, premalignant cells

From: Immortalized, premalignant epithelial cell populations contain long-lived, label-retaining cells that asymmetrically divide and retain their template DNA

  Percentage (%)
Intact (control) gland:Implant group Nulliparous Pregnant
Intact:NSP2 0.20:0.10 0.13:0.01
Intact:NSP3 0.04:0.05 0.08:0.03
Intact:SP3 0:0.09 0.06:0.06
  1. The number of cells expressing an ER-α and 3H-TdR double-label was counted in three random fields of view (400× magnification). In addition, the total number of cells in three random fields of view was determined (>1000 cells total counted; 400× magnification). The total number of cells expressing an ER-α and 3H-TdR double-label were then divided by the total number of cells in a field of view, and the percentage of cells expressing an ER-α and 3H-TdR double-label per field of view was obtained.