Skip to main content
Figure 7 | Breast Cancer Research

Figure 7

From: A novel approach for the generation of genetically modified mammary epithelial cell cultures yields new insights into TGFβ signaling in the mammary gland

Figure 7

Smad dependency of induction of molecular markers of epithelial-to-mesenchymal transition and of MMP-9 by TGFβ. (a) Immunofluorescent staining for E-cadherin and F-actin following 48 hours with or without treatment with 2 ng/ml transforming growth factor beta (TGFβ). (b) Quantitative RT-PCR determination of mRNA levels for key transcriptional regulators of EMT under the same treatment conditions as (a). All data are normalized to the no TGFβ condition for each genotype. *P < 0.05 for the difference between the TGFβ-treated and control conditions. (c) Quantitative RT-PCR for fibronectin mRNA. Data normalized to the wildtype (WT) genotype, no TGFβ treatment. *P < 0.05 for the difference between the TGFβ-treated and control conditions. (d) Gelatin zymography of cell-conditioned medium following 48 hours with or without treatment with 2 ng/ml TGFβ. Medium indicates medium alone control; MMP-2 is present in the serum contained in the culture medium.

Back to article page