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Figure 6 | Breast Cancer Research

Figure 6

From: A novel approach for the generation of genetically modified mammary epithelial cell cultures yields new insights into TGFβ signaling in the mammary gland

Figure 6

Exogenous Smad2 or Smad3 expression restores TGFβ-mediated invasion in immortalized mammary epithelial cells lacking Smad. (a) Adenoviral expression of Smad2 in Smad2 null cells, or of Smad3 in Smad3 null cells, restores sensitivity to transforming growth factor beta (TGFβ)-induced invasion. (b) Adenoviral expression of Smad3 in Smad2 null cells, or of Smad2 in Smad3 null cells, largely restores sensitivity to TGFβ-induced invasion. All values normalized to wildtype (WT) control values. Results are the mean ± standard deviation of three determinations. *P < 0.05 for the difference between specified pairwise comparisons. Western blot analysis of Smad expression in adenovirally transduced immortalized mammary epithelial cells (IMECs) is shown for each experiment. β-actin used as a loading control. NS, not significant.

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Breast Cancer Research

ISSN: 1465-542X

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