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Figure 4 | Breast Cancer Research

Figure 4

From: Differential interleukin-6/Stat3 signaling as a function of cellular context mediates Ras-induced transformation

Figure 4

3-D morphogenic assays of MCF10A-Ras cells display IL-6/Jak/pStat3 signaling which regulate E-Cadherin levels. (a) MCF10A cells were grown in Matrigel as were MCF10A-Ras cells which were stained for pStat3 and Dapi by immunofluorescence treated with DMSO control (Ras-C), P6 (a pan-Jak inhibitor) or a blocking antibody to IL-6 (α-IL-6). (b) MCF10A-Ras cells expressing control (HRas-C) or Stat3 shRNA (S3sh) were grown in Matrigel and stained with Dapi and E-Cadherin (D/E-Cad) by immunofluorescence. (c) MCF10A-Ras cells were grown in matrigel and treated with DMSO (HRas-C), P6 or a blocking antibody to IL-6 (α-IL-6) and stained for Dapi and E-Cadherin (D/E-Cad).

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