Figure 5

Characterization of MaSC-containing and luminal progenitor containing compartments. Mammary epithelial cells isolated as in Figure 4A, were incubated with PE-conjugated anti CD24 Ab, or APC-conjugated anti CD29 Ab and analyzed as described in Methods. Gated: upper left (FVB) and upper second from left (RARα1/KO) for CD24^high/CD29^low (luminal progenitors, upper left square) and for CD24^low/CD29^high (MaSC, lower right square). The third and fourth upper squares-negative controls, cells incubated with conjugated IgGs. The results shown are of one experiment, which was repeated once.