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Figure 1 | Breast Cancer Research

Figure 1

From: An ELISA-based high throughput protein truncation test for inherited breast cancer

Figure 1

Schematic representation of the high-throughput solid-phase protein truncation test (HTS-PTT). The next-generation HTS-PTT uses three different epitope tags - two in tandem at the N-terminus (one capture and one detection tag) and one at the C-terminus (detection tag) - added using specially designed primers (not shown here). The chain truncation mutations are calculated using % C/N (the C- to N-terminal signal ratio of a given sample normalized against known wild-type controls).

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