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Table 1 Specificity of the primary antibodies used, and corresponding subcellular localization and degree of staining observed for ovotestis

From: BRCA1 and BRCA2 protein expressions in an ovotestis of a 46, XX true hermaphrodite

    Staining of ovotestis
    Male germ cells  
Antibodies Specificities Sources and Sertoli cells Female germ cells
Anti-BRCA1     
   K-18* hBRCA1 (aa 70-89) Santa Cruz Biotechnologies Cyt. (+) Cyt. (+)
   8F7 Fusion protein GST-hBRCA1 GeneTex N (+) Cyt. (+), N (+)
  (aa 341-748)    
   17F8 Fusion protein GST-hBRCA1 GeneTex N (+) Cyt. (+)
  (aa 762-1315)    
   66036N* hBRCA1 (aa 2-20) Pharmingen Cyt. (+), N (+) Cyt. (+)
   66046N* hBRCA1 (aa 768-793) Pharmingen Cyt. (+) Cyt. (+)
   66056N* hBRCA1 (aa 1847-1863) Pharmingen Cyt. (+) Cyt. (++)
Anti-BRCA2     
   66066E* hBRCA2 (aa 1323-1346) Pharmingen Cyt. (+) Cyt. (+)
   66076E* hBRCA2 (aa 2586-2600) Pharmingen Cyt. (+) Cyt. (+)
   5F6 (clone 1H3) Fusion protein GST-hBRCA2 GeneTex Cyt. (+) Cyt. (+), N (+)
  (aa 2336-2478)    
   3E6 Fusion protein GST-hBRCA2 GeneTex Cyt. (+) Cyt. (+)
  (aa 188-563)    
  1. *Rabbit polyclonal antibodies and mouse monoclonal antibodies. aa, Amino acid; Cyt., cytoplasm; N, nuclear; (+) intensive; (++), very intensive.