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Table 1 Specificity of the primary antibodies used, and corresponding subcellular localization and degree of staining observed for ovotestis

From: BRCA1 and BRCA2 protein expressions in an ovotestis of a 46, XX true hermaphrodite

   

Staining of ovotestis

   

Male germ cells

 

Antibodies

Specificities

Sources

and Sertoli cells

Female germ cells

Anti-BRCA1

    

   K-18*

hBRCA1 (aa 70-89)

Santa Cruz Biotechnologies

Cyt. (+)

Cyt. (+)

   8F7

Fusion protein GST-hBRCA1

GeneTex

N (+)

Cyt. (+), N (+)

 

(aa 341-748)

   

   17F8

Fusion protein GST-hBRCA1

GeneTex

N (+)

Cyt. (+)

 

(aa 762-1315)

   

   66036N*

hBRCA1 (aa 2-20)

Pharmingen

Cyt. (+), N (+)

Cyt. (+)

   66046N*

hBRCA1 (aa 768-793)

Pharmingen

Cyt. (+)

Cyt. (+)

   66056N*

hBRCA1 (aa 1847-1863)

Pharmingen

Cyt. (+)

Cyt. (++)

Anti-BRCA2

    

   66066E*

hBRCA2 (aa 1323-1346)

Pharmingen

Cyt. (+)

Cyt. (+)

   66076E*

hBRCA2 (aa 2586-2600)

Pharmingen

Cyt. (+)

Cyt. (+)

   5F6 (clone 1H3)

Fusion protein GST-hBRCA2

GeneTex

Cyt. (+)

Cyt. (+), N (+)

 

(aa 2336-2478)

   

   3E6

Fusion protein GST-hBRCA2

GeneTex

Cyt. (+)

Cyt. (+)

 

(aa 188-563)

   
  1. *Rabbit polyclonal antibodies and mouse monoclonal antibodies. aa, Amino acid; Cyt., cytoplasm; N, nuclear; (+) intensive; (++), very intensive.