Skip to main content
Figure 1 | Breast Cancer Research

Figure 1

From: BRCA1 and BRCA2 protein expressions in an ovotestis of a 46, XX true hermaphrodite

Figure 1

Immunohistochemical localization of BRCA1 and BRCA2 with formalin-fixed and paraffin sections in an ovotestis. (a) Hematoxylin eosin saffron (HES) histology of the ovotestis demonstrating testicular tissue with seminiferous cords (triangle), adjacent to ovarian tissue with primordial follicles (arrow) (× 200). For BRCA1 protein: (b) K-18 antibodies showed cytoplasmic staining of oocytes (arrowhead) surrounded by follicule primordial and cytoplasmic staining of male germ cells (arrow) in seminiferous cords identified by the presence of Sertoli cells inside (× 220); (c) 8F7 antibodies showed predominantly nuclear stainings of Sertoli cells (asterisk) and of oocytes (arrowhead), and cytoplasmic staining was also exhibited in the oocytes (× 450); (d) 17F8 antibodies showed exclusive nuclear staining of Sertoli cells (arrow) (× 470); (e) 17F8 antibodies showed cytoplasmic staining of oocytes (arrow) (× 315); (f) 66046N antibodies showed cytoplasmic staining of oocytes (arrowhead) (× 540); and (g) 66036N antibodies showed cytoplasmic staining of male and female germ cells, and slight nuclear staining in Sertoli cells in the tube (asterisk) (× 385). For BRCA2 protein: (h) 66066E antibodies showed cytoplasmic staining of Sertoli cells (arrowhead) and oocytes (asterisk) (× 545); and (i) 3E6 antibodies showed low cytoplasmic staining of Sertoli cells (asterisk) and female germ cells (arrowhead) (× 660).

Back to article page