Silencing of IKKε leads to reduction of Bcl-2 and cyclin D
in breast cancer cells. The Bcl-2 mRNA level was determined in (a) SK-BR-3 cells and (b) MCF-7 cells using real-time RT-PCR. The relative levels of Bcl-2 mRNA in the scrambled siRNA group were normalized as 100%. Results represented as mean ± standard deviation (n = 3). IKKε, Bcl-2 and cyclin D1 expressions at the protein level were assayed using western blot in IKKε siRNA-transfected (c) SK-BR-3 cells and (d) MCF-7 cells. NC, negative control siRNA.