Exogenous p190B expression does not promote MMTV-Neu induced tumorigenesis by affecting the angiogenic switch. (a) Whole-mount mammary glands from tumor-bearing mice were examined to detect hyperplastic lesions. Representative images are shown with arrows indicating hyperplastic lesions. LN indicates lymph nodes. (b) The number of hyperplastic lesions were quantified in tumor-free mammary glands pairs 2/3 and 4 from tumor-bearing mice (n =17 rtTA/Neu and n =13 p190B mice). No statistical differences were detected (t test, P > 0.05). Error bars represent standard error of the mean. (c) Quantification of CD31 immunostaining in tumor sections from p190B (n =11) and rtTA/Neu (n =13) mice demonstrates that p190B does not affect tumor angiogenesis. An average of eight 400× fields were counted per tumor. No statistical differences were detected (t test, P > 0.05). Error bars indicate standard errors (d) F4/80 immunostaining to detect macrophage infiltration in p190B and rtTA control tumors. F4/80 is shown in red and nuclei are blue. Representative 400× images are shown. (e) Quantification of F4/80 immunostaining demonstrates that there is no difference in macrophage infiltration in p190B transgene expressing tumors as compared to rtTA control tumors. Five 400× fields per tumor (n =7 per genotype) were quantified. No statistical differences were detected (t test, P > 0.05). Error bars indicate the standard error of the mean.